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psmad2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc psmad2
    Psmad2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psmad2+3/Smad2%2F3+XP+Rabbit+mAb/pm41735318-295-132-135
    Average 96 stars, based on 1011 article reviews
    psmad2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:


    Article Title: Epigenetic reprogramming via EZH2 inhibition rescues fibroadipose pathogenesis in secondary lymphedema through activating PPARγ signaling
    Article Snippet: pSMAD2/3 , 8828 , Cell Signaling Technology, Beverly, MA, USA.

    Blocking Assay:

    Article Title: Simvastatin inhibits the immunosuppressive effects of endometrial cancer-associated mesenchymal stem cells through TGF-β2/SMAD2/3 signaling and reduces tumor growth
    Article Snippet: Proteins were loaded into 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA). .. After blocking with 1% BSA for 1 h, the proteins, including the phosphorylated form, were incubated with primary antibodies, targeting TGF-β2 (Abacm, Cambridge, UK), pSMAD2/3 (SMAD2: Ser465/467; SMAD3: Ser423/425, Cell Signaling Technology, Danvers, MA, USA), SMAD2/3 (BD Biosciences, Franklin, NJ, USA), PD-L1 (Abacm, Cambridge, UK), and Actin (Cell Signaling Technology) at 4°C overnight and HRP-conjugated secondary antibodies (GeneTex, Irvine, CA, USA) for 1 h. The DAB substrate and ECL (ThermoFisher) were incubated with target proteins and detected by chemiluminescence. .. Each protein and its phosphorylated form were analyzed and quantified using ImageJ software (version 1.54i, NIH, Bethesda, MD, USA).

    Article Title: Critical Role for Transglutaminase 2 in Scleroderma Skin Fibrosis and in the Development of Dermal Sclerosis in a Mouse Model of Scleroderma
    Article Snippet: Samples were heat denatured under reducing conditions with NuPAGE reducing agent (Invitrogen) and subjected to the NuPAGE electrophoresis system (Invitrogen). .. Protein bands were transferred onto nitrocellulose membranes (GE Healthcare), which were incubated in block buffer (5% nonfat dry milk, 0.1% Tween‐20 [Sigma] in phosphate‐buffered saline) for 1 hour, followed by incubation at 4°C overnight with primary antibodies against TGM2 (IA12, UCB Pharma), α‐SMA (71 ng/mL, Dako; Cat M0851/1A4), Col‐1 (0.4 μg/mL, Millipore; Cat AB78), CCN2 (0.1 μg/mL, Santa Cruz Biotechnology; Cat SC‐14939), TNC2 μg/mL (Proteintech; Cat 67710‐1), Shad 2 (Cell signaling; Cat 5678), pSmad2/3 (Cell signaling; Cat 8828), and GAPDH (0.2 μg/mL, Abcam; Cat Ab8245/6C5), diluted in block buffer. .. Blots were developed by incubation with horseradish peroxidase–conjugated secondary antibodies (1:5000, for mouse ‐Cell Signaling; Cat 7076S, for rabbit ‐Cat 7074S).

    Article Title: Huaier-derived neutral polysaccharide WHPB mitigates renal fibrosis via CSF-1R/PI3K/AKT-mediated FUT8 inhibition.
    Article Snippet: Background: Renal interstitial fibrosis (RIF) is a hallmark of chronic kidney disease (CKD).. Fucosyltransferase 8 (FUT8), which mediates core fucosylation (CF), plays a central role in promoting RIF, though its therapeutic potential remains incompletely explored.. Huaier, a traditional Chinese medicine widely used in the clinical treatment of various tumors, has demonstrated promising potential in mitigating renal injury, highlighting its potential as a treatment to suppress FUT8 expression.

    Incubation:

    Article Title: Simvastatin inhibits the immunosuppressive effects of endometrial cancer-associated mesenchymal stem cells through TGF-β2/SMAD2/3 signaling and reduces tumor growth
    Article Snippet: Proteins were loaded into 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA). .. After blocking with 1% BSA for 1 h, the proteins, including the phosphorylated form, were incubated with primary antibodies, targeting TGF-β2 (Abacm, Cambridge, UK), pSMAD2/3 (SMAD2: Ser465/467; SMAD3: Ser423/425, Cell Signaling Technology, Danvers, MA, USA), SMAD2/3 (BD Biosciences, Franklin, NJ, USA), PD-L1 (Abacm, Cambridge, UK), and Actin (Cell Signaling Technology) at 4°C overnight and HRP-conjugated secondary antibodies (GeneTex, Irvine, CA, USA) for 1 h. The DAB substrate and ECL (ThermoFisher) were incubated with target proteins and detected by chemiluminescence. .. Each protein and its phosphorylated form were analyzed and quantified using ImageJ software (version 1.54i, NIH, Bethesda, MD, USA).

    Article Title: Critical Role for Transglutaminase 2 in Scleroderma Skin Fibrosis and in the Development of Dermal Sclerosis in a Mouse Model of Scleroderma
    Article Snippet: Samples were heat denatured under reducing conditions with NuPAGE reducing agent (Invitrogen) and subjected to the NuPAGE electrophoresis system (Invitrogen). .. Protein bands were transferred onto nitrocellulose membranes (GE Healthcare), which were incubated in block buffer (5% nonfat dry milk, 0.1% Tween‐20 [Sigma] in phosphate‐buffered saline) for 1 hour, followed by incubation at 4°C overnight with primary antibodies against TGM2 (IA12, UCB Pharma), α‐SMA (71 ng/mL, Dako; Cat M0851/1A4), Col‐1 (0.4 μg/mL, Millipore; Cat AB78), CCN2 (0.1 μg/mL, Santa Cruz Biotechnology; Cat SC‐14939), TNC2 μg/mL (Proteintech; Cat 67710‐1), Shad 2 (Cell signaling; Cat 5678), pSmad2/3 (Cell signaling; Cat 8828), and GAPDH (0.2 μg/mL, Abcam; Cat Ab8245/6C5), diluted in block buffer. .. Blots were developed by incubation with horseradish peroxidase–conjugated secondary antibodies (1:5000, for mouse ‐Cell Signaling; Cat 7076S, for rabbit ‐Cat 7074S).

    Article Title: Huaier-derived neutral polysaccharide WHPB mitigates renal fibrosis via CSF-1R/PI3K/AKT-mediated FUT8 inhibition.
    Article Snippet: Background: Renal interstitial fibrosis (RIF) is a hallmark of chronic kidney disease (CKD).. Fucosyltransferase 8 (FUT8), which mediates core fucosylation (CF), plays a central role in promoting RIF, though its therapeutic potential remains incompletely explored.. Huaier, a traditional Chinese medicine widely used in the clinical treatment of various tumors, has demonstrated promising potential in mitigating renal injury, highlighting its potential as a treatment to suppress FUT8 expression.

    Saline:

    Article Title: Critical Role for Transglutaminase 2 in Scleroderma Skin Fibrosis and in the Development of Dermal Sclerosis in a Mouse Model of Scleroderma
    Article Snippet: Samples were heat denatured under reducing conditions with NuPAGE reducing agent (Invitrogen) and subjected to the NuPAGE electrophoresis system (Invitrogen). .. Protein bands were transferred onto nitrocellulose membranes (GE Healthcare), which were incubated in block buffer (5% nonfat dry milk, 0.1% Tween‐20 [Sigma] in phosphate‐buffered saline) for 1 hour, followed by incubation at 4°C overnight with primary antibodies against TGM2 (IA12, UCB Pharma), α‐SMA (71 ng/mL, Dako; Cat M0851/1A4), Col‐1 (0.4 μg/mL, Millipore; Cat AB78), CCN2 (0.1 μg/mL, Santa Cruz Biotechnology; Cat SC‐14939), TNC2 μg/mL (Proteintech; Cat 67710‐1), Shad 2 (Cell signaling; Cat 5678), pSmad2/3 (Cell signaling; Cat 8828), and GAPDH (0.2 μg/mL, Abcam; Cat Ab8245/6C5), diluted in block buffer. .. Blots were developed by incubation with horseradish peroxidase–conjugated secondary antibodies (1:5000, for mouse ‐Cell Signaling; Cat 7076S, for rabbit ‐Cat 7074S).

    Control:

    Article Title: Huaier-derived neutral polysaccharide WHPB mitigates renal fibrosis via CSF-1R/PI3K/AKT-mediated FUT8 inhibition.
    Article Snippet: Background: Renal interstitial fibrosis (RIF) is a hallmark of chronic kidney disease (CKD).. Fucosyltransferase 8 (FUT8), which mediates core fucosylation (CF), plays a central role in promoting RIF, though its therapeutic potential remains incompletely explored.. Huaier, a traditional Chinese medicine widely used in the clinical treatment of various tumors, has demonstrated promising potential in mitigating renal injury, highlighting its potential as a treatment to suppress FUT8 expression.



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